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CASP3, CASP7, CASP8, CASP9 and CASP10 mRNA levels (A-E), BCL2, BAX and BID mRNA levels (F-H) and CDKN1A, CDKN1B, TNFα, <t>XIAP</t> and BIRC5 mRNA levels (I-M) are shown. Values are expressed on a logarithmic scale (Log 2) with geometric mean±geometric standard deviation. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. BCL2 mRNA levels were significantly higher in AN-LSCs compared to LSCs (p = 0.0291). In LSCs, BCL2 mRNA level significantly decreased (p = 0.0102) and CDKN1B level significantly increased using 75 µM CoCl 2 treatment. In addition, in AN-LFCs, 75 µM CoCl₂ treatment significantly decreased CASP3, CASP9 and BCL2 mRNA levels (p = 0.0494; p = 0.0414; p = 0.0218).
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CASP3, CASP7, CASP8, CASP9 and CASP10 mRNA levels (A-E), BCL2, BAX and BID mRNA levels (F-H) and CDKN1A, CDKN1B, TNFα, XIAP and BIRC5 mRNA levels (I-M) are shown. Values are expressed on a logarithmic scale (Log 2) with geometric mean±geometric standard deviation. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. BCL2 mRNA levels were significantly higher in AN-LSCs compared to LSCs (p = 0.0291). In LSCs, BCL2 mRNA level significantly decreased (p = 0.0102) and CDKN1B level significantly increased using 75 µM CoCl 2 treatment. In addition, in AN-LFCs, 75 µM CoCl₂ treatment significantly decreased CASP3, CASP9 and BCL2 mRNA levels (p = 0.0494; p = 0.0414; p = 0.0218).

Journal: PLOS One

Article Title: Altered Bcl-2/Caspase signaling and hypoxia-induced apoptosis in primary human aniridia limbal stromal cells, in CoCl 2 mediated hypoxic stress, in vitro

doi: 10.1371/journal.pone.0328157

Figure Lengend Snippet: CASP3, CASP7, CASP8, CASP9 and CASP10 mRNA levels (A-E), BCL2, BAX and BID mRNA levels (F-H) and CDKN1A, CDKN1B, TNFα, XIAP and BIRC5 mRNA levels (I-M) are shown. Values are expressed on a logarithmic scale (Log 2) with geometric mean±geometric standard deviation. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. BCL2 mRNA levels were significantly higher in AN-LSCs compared to LSCs (p = 0.0291). In LSCs, BCL2 mRNA level significantly decreased (p = 0.0102) and CDKN1B level significantly increased using 75 µM CoCl 2 treatment. In addition, in AN-LFCs, 75 µM CoCl₂ treatment significantly decreased CASP3, CASP9 and BCL2 mRNA levels (p = 0.0494; p = 0.0414; p = 0.0218).

Article Snippet: XIAP Polyclonal antibody , XIAP , Proteintech , #23453–1-AP , Rabbit , 1:100.

Techniques: Standard Deviation

Data represent mean±SD from three independent experiments. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. p21, p27, XIAP, TNF-α, and Survivin protein levels are shown. Representative histograms show primary antibody staining in green, while staining with the corresponding secondary antibody alone (negative control) is shown in red. MFI: mean fluorescence intensity, normalized to the secondary antibody control. In AN-LSCs, p21 and p27 protein levels were significantly higher (p = 0.0003; p = 0.0164) and XIAP and Survivin protein levels were significantly lower (p = 0.0003; p = 0.0074), than in LSCs, using flow cytometry. In addition, using ELISA, in cell culture supernatant of AN-LSCs, TNF-α protein levels were significantly higher following 75 µM CoCl 2 treatment, than in untreated controls (p = 0.0251).

Journal: PLOS One

Article Title: Altered Bcl-2/Caspase signaling and hypoxia-induced apoptosis in primary human aniridia limbal stromal cells, in CoCl 2 mediated hypoxic stress, in vitro

doi: 10.1371/journal.pone.0328157

Figure Lengend Snippet: Data represent mean±SD from three independent experiments. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. p21, p27, XIAP, TNF-α, and Survivin protein levels are shown. Representative histograms show primary antibody staining in green, while staining with the corresponding secondary antibody alone (negative control) is shown in red. MFI: mean fluorescence intensity, normalized to the secondary antibody control. In AN-LSCs, p21 and p27 protein levels were significantly higher (p = 0.0003; p = 0.0164) and XIAP and Survivin protein levels were significantly lower (p = 0.0003; p = 0.0074), than in LSCs, using flow cytometry. In addition, using ELISA, in cell culture supernatant of AN-LSCs, TNF-α protein levels were significantly higher following 75 µM CoCl 2 treatment, than in untreated controls (p = 0.0251).

Article Snippet: XIAP Polyclonal antibody , XIAP , Proteintech , #23453–1-AP , Rabbit , 1:100.

Techniques: Staining, Negative Control, Fluorescence, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture